First report of Sugarcane streak mosaic virus in Iran

Identification of wheat streak mosaic virus in iran

A type of sugarcane mosaic virus infecting maize,surghum and johnsongrass in iran

Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana

Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...

Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles.

Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by s...

Hot Water Treatment of Cutting-cane Infected with Sugarcane Streak Mosaic Virus (scsmv)

The thermal inactivation point (TIP) of SCSMV was determined by heating the infected plant sap for 10 min at 50oC, 55oC, 60oC, and 65oC. The sap was inoculated mechanically on indicator plant Dactylactonium aegypticum. The virus was detected by a reverse transcription polymerase chain reaction (RT-PCR) using specific primer to amplify a part of the coat protein (CP) gene. The SCSMV was detected...